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ISBN 13: 9780521084895
Add a comment. Pignatti [ 17 ] described beside M.
Darlington and Wylie [ 4 ] presented the haploid chromosome number for M. Lemon balm is of increasing importance, resulting in rising growth area and investigations for improved new varieties. Better winter hardiness, higher content of essential oil and higher yield of M. Adaptation of technologies for acceleration of the breeding process using doubled haploid lines has been initiated. Therefore, the knowledge on the genome structure and number of chromosomes in connection with the taxonomical structure of M. In order to determine the ploidy level of 40 accessions and the chromosome number of the haploid M.
Genome size determination by flow cytometry of M. Unlike this result in some other genera e.
In accessions showing six or eight hybridization signals, the intensity of rDNA signals varied. The same was true for 5S rRNA sites. Metaphases of genotypes showing eight rDNA signals for each marker, one chromosome pair displayed a strong and one pair a weak hybridization signal Fig. Later on, Tutin et al. The reports of Heidari et al.
Two scenarios regarding the origin of triploid balm can be postulated: an unreduced gamete of a diploid plant formed triploid offspring after fertilization with a haploid gamete. Alternatively, a tetraploid parent hybridized with diploid parent and formed triploid offspring. The plant phenotype of the four triploid accessions was characterized. The size of leaves 7. The internodes are longer 9.
Triploid accessions had very good cold resistance and regenerated faster after winter and harvesting cuts results not shown. The colour of leaves was bluish to greyish green in comparison to green leaves of ssp. Young leaves of triploid accessions have indumenta similar to ssp. Adult leaves of triploid accessions are more similar to ssp. Stems of triploid accessions are greyish- or whitish-tomentose beneath with similarity to ssp.
The triploid accessions are not lemon-scented. They had a soap-like, nauseating scent. These plants had an inconspicuous formation of flowers but do not produce any seed, neither under conditions of isolation nor by open pollination, likely due to meiotic problems.
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These first time described triploid accessions were propagated by cuttings and are cytologically and morphologically stable for at least six years. This is the first characterization of triploids in M.
These triploid accessions are sterile but cytologically and morphologically stable. The length and width of the leaves and the length of internodes exceeded the comparable data for diploid accessions but are not significant for all characters. For exact origin analysis of triploids as well as the characterization of allotetraploid or autotetraploid character of tetraploids analysis of meiotic chromosome pairing is necessary. Chromosome specific molecular markers would open the chance to ascertain the level of similarity of homoeologous groups of chromosomes. This is a prerequisite for better characterization of phylogenetic distance of M.
A set of 40 accessions of M.
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All IPK accessions were grown from seeds whereas all LfL accessions were propagated by cuttings starting with a single plant. Radish Raphanus sativus L. Measuring of relative DNA amount of nuclei occurred by flow cytometry Facs calibur, Becton Dickinson, BD with a red fluorescence laser as basis for detection of ploidy level. For reference, radish was measured in separate sample after five samples of balm. For vegetative multiplied accessions LfL , root tips from potted plants were used. In contrast to Pan et al. After washing with aqua dest.
The 18S Pseudocoloration and mergence of images were done with software of the Isis program Metasystems. Characterization of geographically isolated accessions in five Alstroemeria L. Pl Syst Evol.itlauto.com/wp-includes/mspy/3428-camra-de-surveillance.php
Melisse Melissa officinalis L. Balm Melissa officinalis L. In: Hoppe B, editor. Bernburg: Saluplanta; Deutsches Arzneibuch. Stuttgart: Deutscher Apothekerverlag; Chromosome atlas of flowering plants. Davis PH.
Black Swallowwort – CISMA
Flora of turkey and the east Aegean islands, Vol. Edinburg: University Press; European Union.
Pharmacopoea Europaea , 8th Ed. Fuchs J, Schubert I. Localization of seed protein genes on metaphase chromosomes of Vicia faba via fluorescence in situ hybridization. Chromosom Res. Merrill and conservation of the 5S rDNA repeat structure in higher plants. Berlin, Heidelberg, New York: Springer; In: Kubitzki K, editor. The families and genera of vascular plants. Heidari P, Mehrabi AA.
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